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Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method 下载免费PDF全文
Yu Pan Tao Shen Ji Zhang Yan‐Li Zhao Yuan‐Zhong Wang Wan‐Yi Li 《Biomedical chromatography : BMC》2015,29(1):87-96
Many species from genus Gentiana (Gentianaceae) have a long history of applications as folk medicines in the world. A simple rapid UPLC‐UV‐MS/MS method has been developed and validated for the simultaneous determination of six index constituents (gentiopicroside, swertiamarin, loganic acid, sweroside, mangiferin and ferulic acid) from the four ethnomedicines (G. rigescens Franch. ex Hemsl., G. rhodantha Franch. ex Hemsl., G. scabra Bunge and G. farreri Balf. f.). The UPLC analysis was performed on Shim‐Pack XR‐ODS III (150 × 2.0 mm, 2.2 µm). The mobile phase was consisted of acetonitrile–0.1% formic acid water using gradient elution. The wavelength 242 nm was chose for the four iridoids as well as mangiferin and 320 nm was set for ferulic acid. Mass spectrometry was applied for identification and quantification for analytes with low concentration. All the regression equations revealed a good linear relationship (R2 > 0.9993). Accuracy and precision were all within the required limits. The chromatogram fingerprints analysis combined with principal component analysis showed the similarity values of the four species were <0.788 while the similarity values of G. scabra Bunge and G. rigescens Franch. ex Hemsl. were >0.993, which provided reasonable foundation for utilization and clinical application of the four ethnomedicines. This developed method appears to be a useful tool for quality control of the four ethnomedicines. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
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源于真菌的天然产物细胞松弛素类化合物(cytochalasins)是微生物研究的热点领域之一.该类化合物结构复杂多样,核磁共振(NMR)波谱鉴定困难.Chaetoglobosin F是分离自五加科药用植物三七(Panax notoginseng)种子内生真菌Chaetomium globosum中的10-吲哚类细胞松弛素.本文对chaetoglobosin F进行NMR结构解析及数据归属,并总结归纳10-吲哚类细胞松弛素的NMR特征,为该类化合物的深入研究提供参考. 相似文献
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Guang-chao Ma Tie-shan Wang Ji Wang Zhi-jie Ma Shi-biao Pu 《Biomedical chromatography : BMC》2020,34(3):e4739
Allergic rhinitis (AR) negatively affects the healthy lives of many individuals. Most previous studies on AR focused on the expression of cytokines, with only a few analyzing cytokine expression from a metabolomics viewpoint. Therefore, it is worthwhile to study AR at the metabolic level. Consequently, we aimed to identify differential serum biomarkers by metabolomics. In this study, the orthogonal partial least squares discriminant analysis (OPLS-DA) model was applied to characterize the differences in serum samples collected from patients with AR and healthy volunteers. Ten metabolites (except hexadecanoic acid) were found to be altered significantly (p < .05) in the former group, according to results of principal component analysis and OPLS-DA, indicating that these metabolites could be potential biomarkers. MetaboAnalyst 4.0 and pathway enrichment analysis showed that these changes in metabolites mainly involved three pathways, namely, porphyrin and chlorophyll metabolism, arachidonic acid metabolism, and purine metabolism. Our findings may contribute to a better understanding of the potential pathogenesis mechanisms and provide a metabolic evidence for in-depth studies of AR. 相似文献
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AbstractTwo new eremophilane sesquiterpenoides, 6α,9α-dihydroxyeremophilenolide (1), and 1β,10β-dihydroxyeremophilenolide (2), along with ten known eremophilane sesquiterpenoides (3–12) were isolated from the aerial parts of Ligularia dictyoneura (Franch.) Hand.-Mazz. Their structures were elucidated by means of extensive spectroscopic analysis. Compounds 3–6 were assessed for their cytotoxicity against five human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7 and SW-480), and the result showed that they had no activity. 相似文献
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傅里叶变换红外光谱(FTIR)结合偏最小二乘判别分析(PLS-DA)和偏最小二乘回归(PLSR)对不同采收期滇重楼(Paris polyphylla var. yunnanensis)分别进行定性鉴别与定量分析,以期为滇重楼合理采收和鉴别评价提供科学依据。采集46份不同采收期滇重楼样品的红外光谱,对光谱数据进行自动基线校正+纵坐标归一化+自动平滑+小波去噪(WD)预处理后进行PLS-DA分析;采用超高效液相色谱测定样品中重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ的含量,将液相测定数据与红外光谱数据进行拟合,经自动基线校正+纵坐标归一化+自动平滑+一阶求导+正交信号校正(OSC)优化处理后,建立滇重楼中重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ的快速预测模型。结果显示,(1)原始红外光谱中主要吸收区域在950~700,1 200~950,1 800~1 500和2 800~3 500 cm-1附近。(2)PLS-DA得分图可准确区分不同采收期滇重楼样品。(3)液相数据显示重楼总皂苷含量随着年限的增加先成倍增加,再逐渐减少,最后呈现缓慢增加的趋势。(4)重楼总皂苷含量定量模型的预测值与真实值间无显著性差异,表明模型预测效果好。FTIR结合化学计量学可准确区分不同采收期滇重楼并快速预测其皂苷含量,为不同采收期滇重楼的鉴别和皂苷含量预测提供一种新方法,同时为确定滇重楼的最佳采收期提供参考依据。 相似文献